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1.
Sci Rep ; 13(1): 14678, 2023 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-37673935

RESUMO

This paper proposes a functionally reconfigurable integrated structure of a Fabry-Perot (FP) antenna and wideband liquid absorber. First, a two-layer partial reflecting surface (PRS) has been designed. Then, a patch antenna is used to act as the source antenna. By combining the source antenna with the PRS, an FP antenna has been designed. What's more, taking full advantage of the reflective properties of PRS, a liquid broadband absorber is then designed. Last, the integrated structure with two functions has been realized. It can be used as the FP antenna or the liquid absorber through the extraction and injection of ethanol. In this way, it is effective to switch between stealth and detection states which can be used in different electromagnetic environments. The PRS is elaborately tailored to serve as both a component of the FP antenna and the metal ground of the broadband liquid absorber. Then the integrated structure is realized by combining the FP antenna with the liquid absorber. The PRS is composed of patches on the top layer of the substrate and the square loop on the bottom. The liquid absorber is composed of a 3-D printed container, 45% ethanol layer and the PRS is used to serve as the metal ground. The formula of Mie resonance theory has been extended and used to design the liquid absorber. The gain of the antenna is 19.7 dBi when the ethanol is extracted. When the ethanol is injected, a wideband liquid absorber is achieved. The absorption band (S11 < - 10 dB) ranges from 4 to 18 GHz. The absorption bandwidth is over 133%. The monostatic RCS reduction bands of the structure with ethanol range from 4 to 18 GHz and the average RCS reduction is 28.4 dBsm. The measured and simulated results are in good agreement.

2.
Plant Biotechnol J ; 21(2): 342-353, 2023 02.
Artigo em Inglês | MEDLINE | ID: mdl-36278914

RESUMO

The widespread application of isoproturon (IPU) can cause serious pollution to the environment and threaten ecological functions. In this study, the IPU bacterial N-demethylase gene pdmAB was transferred and expressed in the chloroplast of soybean (Glycine max L. 'Zhonghuang13'). The transgenic soybeans exhibited significant tolerance to IPU and demethylated IPU to a less phytotoxic metabolite 3-(4-isopropylphenyl)-1-methylurea (MDIPU) in vivo. The transgenic soybeans removed 98% and 84% IPU from water and soil within 5 and 14 days, respectively, while accumulating less IPU in plant tissues compared with the wild-type (WT). Under IPU stress, transgenic soybeans showed a higher symbiotic nitrogen fixation performance (with higher total nodule biomass and nitrogenase activity) and a more stable rhizosphere bacterial community than the WT. This study developed a transgenic (TS) soybean capable of efficiently removing IPU from its growing environment and recovering a high-symbiotic nitrogen fixation capacity under IPU stress, and provides new insights into the interactions between rhizosphere microorganisms and TS legumes under herbicide stress.


Assuntos
Glycine max , Solo , Biodegradação Ambiental , Glycine max/genética , Glycine max/metabolismo , Compostos de Fenilureia/metabolismo
3.
Appl Environ Microbiol ; 87(11)2021 05 11.
Artigo em Inglês | MEDLINE | ID: mdl-33771783

RESUMO

1-Naphthol, a widely used raw material for organic synthesis, is also a well-known organic pollutant. Due to its high toxicity, 1-naphthol is rarely used by microorganisms as the sole carbon source for growth. In this study, catabolism of 1-naphthol by Sphingobium sp. strain B2 was found to be greatly enhanced by additional supplementation with primary carbon sources (e.g., glucose, maltose, and sucrose), and 1-naphthol was even used as the carbon source for growth when strain B2 cells had been preinduced by both 1-naphthol and glucose. A distinct two-component flavin-dependent monooxygenase, NdcA1A2, was found to be responsible for the initial hydroxylation of 1-naphthol to 1,2-dihydroxynaphthalene, a more toxic compound. Transcriptional levels of ndcA1A2 genes were significantly upregulated when strain B2 cells were cultured with both 1-naphthol and glucose compared to cells cultured with only 1-naphthol or glucose. Two transcriptional regulators, the activator NdcS and the inhibitor NdcR, were found to play key roles in the synergistic regulation of the transcription of the 1-naphthol initial catabolism genes ndcA1A2IMPORTANCE Cometabolism is a widely observed phenomenon, especially in the field of microbial catabolism of highly toxic xenobiotics. However, the mechanisms of cometabolism are ambiguous, and the roles of the obligately coexisting growth substrates remain largely unknown. In this study, we revealed that the roles of the coexisting primary carbon sources (e.g., glucose) in the enhanced catabolism of the toxic compound 1-naphthol in Sphingobium sp. strain B2 were not solely because they were used as growth substrates to support cell growth but, more importantly, because they acted as coinducers to interact with two transcriptional regulators, the activator NdcS and the inhibitor NdcR, to synergistically regulate the transcription of the 1-naphthol initial catabolism genes ndcA1A2 Our findings provide new insights into the cometabolic mechanism of highly toxic compounds in microorganisms.


Assuntos
Proteínas de Bactérias/genética , Regulação Bacteriana da Expressão Gênica , Oxigenases de Função Mista/genética , Naftóis/metabolismo , Sphingomonadaceae/genética , Proteínas de Bactérias/metabolismo , Oxigenases de Função Mista/metabolismo , Sphingomonadaceae/enzimologia
4.
Sci Rep ; 10(1): 17843, 2020 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-33082468

RESUMO

In this paper, a metasurface composed of 3-bit coding linear polarization conversion elements and its application to RCS reduction of the patch antenna is intensively studied. At first, 3-bit coding metasurface are constructed by a sequence of eight coded unit cells, which have a similar cross-polarized reflected amplitude response and gradient reflected phase responses covering 0-2π, respectively. Equivalent circuit models of these unit cells are created to describe their electrical behavior for the two linear incident polarizations at the same time. Then, a patch antenna is integrated on the 3-bit metasurface, of which the elements are placed with a 2-dimensional linear coding sequence. The metal square ring is set around the patch antenna to protect it from the disturbance of metasurface. Both the simulation and experiment results demonstrate that the designed metasurface can primarily reduce the antenna RCS at a broadband, while the antenna performances are not degraded significantly.

5.
J Colloid Interface Sci ; 571: 412-418, 2020 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-32220681

RESUMO

TiO2 is a popular photocatalyst due to its low cost and easy availability. Herein, we for the first time synthesized carbon coated TiO2 nano-dendrites (C-TiO2 NDs) using a simple hydrothermal method without high-temperature sintering, in which citric acid is used as an adjuvant. This unique dendritic structure consisting of a single small hexagonal piece greatly increases the BET specific area (116.386 m2 g-1) and pore size (0.418 cm3 g-1), increasing the photocatalytic active site and facilitate efficient capture of light. Besides, compared with the TiO2 nanobelts (NBs) prepared by sintering method, the surface of C-TiO2 NDs prepared by hydrothermal method with citric acid are coated with carbon layers, which make C-TiO2 NDs exhibit stronger photocatalytic hydrogen evolution performance under simulated solar light irradiation due to the presence of carbon coatings promoting electron-hole separation and absorbing NIR light.

6.
Int J Syst Evol Microbiol ; 69(11): 3443-3447, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31436521

RESUMO

A Gram-stain-negative bacterial strain, designated JW-3T, was isolated from a soil sample collected from farmland in Yantai, Shandong Province, PR China. Cells of strain JW-3T are motile rods and strictly aerobic, showing catalase- and oxidase-positive reactions. Strain JW-3T could grow at 16-37 °C (optimum, 30 °C), at pH 6.0-9.0 (pH 7.0) and in the presence of 0-1 % (w/v) NaCl (0.5 %, in Luria-Bertani broth). The major fatty acids were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c; 35.5 %), iso-C16 : 0 (16.7 %) and C12 : 0 (10.8 %). The major respiratory quinone was ubiquinone-8 (Q8). The polar lipids of strain JW-3T consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, four unidentified phospholipids, two unidentified lipids, two unidentified glycolipids and a partial unidentified aminophospholipid. Strain JW-3T was most closely related to Steroidobacter agariperforans KA5-BT with 97.67 % 16S rRNA gene sequence similarity. Results of phylogenetic analyses, based on 16S rRNA gene sequencing, showed that strain JW-3T forms a distinct phylogenic lineage within the genus Steroidobacter of the family Sinobacteraceae. The DNA G+C content of strain JW-3T was 62.57 mol%, based on its draft genome sequence. Average nucleotide identity values and digital DNA-DNA hybridization values for draft genomes, between strain JW-3T and strain KA5-BT, were 84.54 and 30.80 %, respectively. Based on its phenotypic, chemotaxonomic and molecular features, and DNA-DNA hybridization results, strain JW-3T represents a novel species of the genus Steroidobacter, for which the name Steroidobactersoli sp. nov. is proposed. The type strain is JW-3T (=CCTCC AB 2018184T=KCTC 62820T).


Assuntos
Fazendas , Gammaproteobacteria/classificação , Filogenia , Microbiologia do Solo , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Gammaproteobacteria/isolamento & purificação , Glicolipídeos/química , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Ubiquinona/química
7.
Int J Syst Evol Microbiol ; 69(6): 1783-1788, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30973320

RESUMO

An aerobic bacterial strain, designated XJ-2T, was isolated from a soil sample collected from Gurbantunggut Sandy Desert in PR China. Cells of strain XJ-2T were Gram-stain-negative, non-motile, rod-shaped and non-spore-forming. The new isolate grew well at 10-37 °C (optimum, 28-30 °C), pH 6.0-11.0 (pH 7.0) and 0-1 % (w/v) NaCl (0 %). The 16S rRNA gene sequence of strain XJ-2T showed the highest similarity to that of Chitinophaga rhizosphaerae T16R-86T (99.0 %), followed by Chitinophaga barathri YLT18T (97.0 %), Chitinophagahumicola Ktm-2T (96.7 %) and Chitinophaga niabensis JS13-10T (96.4 %). The major menaquinone of strain XJ-2T was menaquinone 7 and the predominant fatty acids (>5 %) were iso-C15 : 0, C16 : 1ω5c and iso-C17 : 0 3-OH. The polar lipids consisted of phosphatidylethanolamine, an unidentified glycolipid, three unidentified aminolipids and five unidentified lipids. The genome size was 6.33 Mb, comprising 5268 predicted genes with a G+C content of 41.5 mol%. The DNA G+C content was 50.5 mol% based on total genome calculations. The average nucleotide identity and the digital DNA-DNA hybridization values between strain XJ-2T and strain T16R-86T were 79.6 and 22.3 %, respectively. DNA-DNA relatedness between strain XJ-2T and strain YLT18T was 17.0 %. Based on the physiological, biochemical and chemotaxonomic characteristics, strain XJ-2T represents a novel species of the genus Chitinophaga, for which the name Chitinophagadeserti sp. nov. is proposed. The type strain is XJ-2T (KCTC 62443T=CCTCC AB 2018019T).


Assuntos
Bacteroidetes/classificação , Clima Desértico , Filogenia , Microbiologia do Solo , Técnicas de Tipagem Bacteriana , Bacteroidetes/isolamento & purificação , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Fosfatidiletanolaminas/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/química
8.
Opt Express ; 21 Suppl 2: A231-40, 2013 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-23482285

RESUMO

By using optimized transformation function, we research on a minimized scattering nonmagnetic concentrator, which can realize impedance matching at the inner and the outer boundaries. It has been demonstrated that the optimized transformation function method can improve the concentrating performance remarkably. The cylindrical anisotropic shell can be mimicked by radial symmetrical sectors which alternate in composition between two profiles of isotropic dielectrics, and the permittivity in each sector can be properly determined by the effective medium theory. The nonmagnetic concentrator has been validated by full-wave finite element simulations. We can believe that this work will improve the flexibilities for the EM concentrator design.

9.
Appl Opt ; 50(16): 2370-5, 2011 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-21629315

RESUMO

The transmittance of one-dimensional photonic crystals consisting of superconductor and lossless dielectric has been systematically studied through the transfer-matrix method. Obviously, the shift of the photonic bandgap (PBG) becomes more noticeable by adjusting the thicknesses of the dielectric layers than that of superconductor layers. Furthermore, the number of PBGs can be controlled by varying the thicknesses of dielectric layers. Compared to the thicknesses of the dielectric layers, the width of the PBGs is more sensitive to the thicknesses of the superconductor layers. However, the width of the first PBG promptly varies when the thicknesses of the dielectric layers increase from 0 to 40 nm. If the contribution of the normal conducting electrons of the superconductor is nonnegligible, the temperature of the superconductor has no influence on the width of the PBGs. Moreover, the damp coefficient does not affect the PBGs under low-temperature conditions.

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